Evaluation of the InGenius platform for carbapenem and beta-lactam antibiotic-resistant bacteria testing shows great results

As antibiotic resistant bacterial strains are becoming more and more commonly found in hospitals, automated molecular biology solutions will help to tackle the burden of increased testing. The ELITe InGenius full-automation instrument has a wide portfolio of multiplex virus and bacteria testing but it also has several assays for detection of antibiotic-resistant bacteria strains such as Methicillin-resistant Staphylococcus aureus/Staphylococcus aureus (MRSA/SA), carbapenemase-producing Enterobacteriaceae (CRE), and extended-spectrum beta-lactamase (ESBL). Girlich et al. (2018) have evaluated the CRE and ESBL ELITe MGB assays and found an impressive 100% sensitivity and 100% specificity for the CRE ELITe MGB kit and 100% sensitivity and 96,6% specificity for the ESBL ELITe MGB Kit.

The CRE ELITe MGB results were also compared to the results by the French National Reference Center (FNRC), which had identified carbapenem-resistant Enterobacteriaceae genes from the sample pool in France during years 2012-2015. Out of that sample pool, FNRC had confirmed 3320 samples positive for CRE. Based on those findings, researchers expected to have sensitivity of 100% and specificity of 99,58% for CRE ELITe MGB kit in best scenario. Therefore, the final 100% sensitivity and 100% specificity values are excellent.

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